File Name: coding and noncoding sequences ppt to .zip
Comparative sequence analysis has facilitated the discovery of protein coding genes and important functional sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of sequences of relatively closely related species. We tested the use of comparative DNA sequence analysis to aid identification of promoter regulatory elements, nonprotein-coding RNA genes, and small protein-coding genes by surveying random DNA sequences of several Saccharomyces yeast species, with the goal of learning which species are best suited for comparisons with S. We also determined the DNA sequence of a few specific promoters and RNA genes of several Saccharomyces species to determine the degree of conservation of known functional elements within the genome.
Regulatory ncRNAs are more interesting from an epigenetic point of view as they are involved in the modification of other RNAs. They can be further classified into the following:. Learn more about our new multiplex miRNA assays. Their main role is the silencing of transposable elements during germ line development. The majority of non-coding RNAs belong to this group. Many of the lncRNAs are subject to splicing, polyadenylation and other post-transcriptional modifications, and can be classified according to their proximity to protein coding genes.
LincRNAs are involved in epigenetic gene silencing, such as the role of Xist X-inactive specific transcript and in tumor development by promoting expression of genes involved in metastasis and angiogenesis. These two types of ncRNAs have been described recently and their roles are still unclear.
Enhancer RNAs are non-coding transcripts with an average of nt ranging between 0. It has been postulated that eRNAs function as transcriptional activators. PARs are non-coding transcripts that range from 16 nt to nt, and they are generally expressed near the transcription start site or in upstream elements of the promoter. Most of the PARs are associated with highly expressed genes, but they are weakly expressed and with short half-lives.
It has been postulated that PARs are connected with transcriptional activation and repression. Interest in RNA-protein interactions is increasing as we begin to appreciate the role of RNA, not just in well-established processes such as transcription, splicing, and translation, but also in newer fields such as RNA interference and epigenetic regulation of gene expression by ncRNAs.
Continue Continue. Epigenetics guides. DNA methlyation. Histone modifications. Epigenetics resources. Conference calendar. Products for your research. Panels for miRNA research. RNA products. Browse antibodies for CLIP. Analysis of RNA-protein interactions Interest in RNA-protein interactions is increasing as we begin to appreciate the role of RNA, not just in well-established processes such as transcription, splicing, and translation, but also in newer fields such as RNA interference and epigenetic regulation of gene expression by ncRNAs.
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The comparison of complete genomes has revealed surprisingly large numbers of conserved non-protein-coding CNC DNA regions. However, the biological function of CNC remains elusive. CNC differ in two aspects from conserved protein-coding regions. They are not conserved across phylum boundaries, and they do not contain readily detectable sub-domains. Here we characterize the persistence length and time of CNC and conserved protein-coding regions in the vertebrate and insect lineages.
Finding DNA sequences that show significant signs of positive or negative selection Regulatory regions ~ conserved non-coding sequences. ▫ Pennachio etc.
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