gram positive and gram negative bacteria difference pdf

Gram positive and gram negative bacteria difference pdf

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Introduction

Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma

Staining and Identification

Retain crystal violet dye and stain blue or purple. Can be decolorized to accept counterstain safranin and stain pink or red 2 Cell Wall Cell Wall is nm thick. Cell Wall is nm thick. The wall is wavy.

Introduction

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Show related SlideShares at end. WordPress Shortcode. Published in: Education. Full Name Comment goes here. Are you sure you want to Yes No. Pragya Prabha. Prachi Verma. Show More. No Downloads. Views Total views. Actions Shares. No notes for slide. Christian Gram, a Danish Physician in developed a staining technique to distinguish two types of bacteria. The two categories of bacteria based on gram staining are Gram positive bacteria and Gram negative bacteria.

Bacteria are first stained with crystal violet or gentian violet. All bacterial cells will stain blue or purple colour with crystal violet solution. Gram negative bacteria are later stained with safranin or fuchsin for observation under microscope. Gram negative bacteria after safranin or fuchsin staining colour red or pink colour. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls by detecting the properties of peptidoglycan. Gram staining method is useful in differentiating majority of bacterial species into two broad categories.

Even though all bacterial species cannot be differentiated based on gram staining technique, this method has immense application in clinical diagnostics and biological researches.

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Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma

Gram-positive bacteria are bacteria with thick cell walls. In a Gram stain test , these organisms yield a positive result. Though both groups of bacteria can cause disease, they require different treatments. If you have a bacterial infection, the Gram stain will determine what kind of medication you need. Read on to learn about gram-positive bacteria and their associated diseases, along with typical treatments.

A gram-negative bacteria is a prokaryotic cell with a cell wall containing small amounts of peptidoglycan and an outer membrane containing lipopolysaccharides, proteins, and other complex macromolecules. The distinction between gram-positive and gram-negative bacteria is based on a differential stain, the Gram stain, which depends on the structure of the cell wall see cell wall. After Gram staining, gram-positive bacteria appear purple and gram-negative bacteria appear red. This difference in reaction to the Gram stain arises because of differences in the cell wall structure which promote the retention of the purple dye, crystal violet, in the gram-positive bacteria after decoloring with ethanol, while in the gram-negative, the stain is not retained. The Gram stain is one of the most useful staining procedures in microbiology. In addition to the peptidoglycan, gram-negative bacteria contain an additional wall layer, the outer membrane.

Staining and Identification

In bacteriology , gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick peptidoglycan layer in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells, making the cell wall more porous and incapable of retaining the crystal violet stain. Their peptidoglycan layer is much thinner and sandwiched between an inner cell membrane and a bacterial outer membrane , causing them to take up the counterstain safranin or fuchsine and appear red or pink.

Show Caption Hide Anthrax gram stain. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with. Alternatively, Gram negative bacteria stain red, which is attributed to a thinner peptidoglycan wall, which does not retain the crystal violet during the decoloring process.

Mar 12, 21 PM. What do plasma cells do? Also known as plasma B cells, plasma cells are terminally differentiated B lymphocytes. Read more here.

2 comments

  • Eliot R. 19.04.2021 at 02:49

    Gram-negative bacteria more pathogenic rather than bacteria gram-positive bacteria [11]. Gram-negative bacteria are characterized by cell wall.

    Reply
  • YerimГ©n V. 20.04.2021 at 20:46

    What is the gram-negative bacteria and its cell wall structure? ‐ What are the main differences between gram positive and gram negative bacteria?

    Reply

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